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sendai virus strain cantell  (ATCC)


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    ATCC sendai virus strain cantell
    Sendai Virus Strain Cantell, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sendai virus strain cantell/product/ATCC
    Average 93 stars, based on 37 article reviews
    sendai virus strain cantell - by Bioz Stars, 2026-03
    93/100 stars

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    Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.

    Journal: medRxiv

    Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens

    doi: 10.64898/2026.02.06.26345651

    Figure Lengend Snippet: Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.

    Article Snippet: Extracted RNA (8μl) was treated with DNAse, as recommended, then spiked with three reverse transcription/amplification controls (1μl each, containing approximately 10 4 genome copies each of Zika virus (ATCC-VR-1838DQ), murine respirovirus (ATCC-VR-907DQ) and orthoreovirus (ATCC-VR-824DQ) (ATCC Manassas, Virginia, USA). cDNA synthesis was primed randomly using the 3’ terminal N 9 segment of custom oligonucleotide primers (5’-GAT-GAT-AGT-AGG-GCT-TCG-TCA-CNN-NNN-NNN N-3’; Integrated DNA Technologies, Leuven, Belgium), final concentration 5μM.

    Techniques: Virus, cDNA Synthesis, Extraction, Control, Biomarker Discovery